Plasmid
Part:BBa_I745002:Design
Designed by: Trono lab Group: iGEM07_Princeton (2007-10-26)
pTHM
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5392
Illegal XbaI site found at 5884
Illegal SpeI site found at 4329
Illegal PstI site found at 2433
Illegal PstI site found at 2938
Illegal PstI site found at 5386 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5392
Illegal NheI site found at 4298
Illegal NheI site found at 4302
Illegal NheI site found at 6143
Illegal SpeI site found at 4329
Illegal PstI site found at 2433
Illegal PstI site found at 2938
Illegal PstI site found at 5386
Illegal NotI site found at 1144 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5392
Illegal BglII site found at 474
Illegal BglII site found at 2687
Illegal BglII site found at 4280
Illegal BglII site found at 4985
Illegal BglII site found at 5719
Illegal BglII site found at 9131
Illegal BamHI site found at 5077
Illegal XhoI site found at 3086
Illegal XhoI site found at 3482
Illegal XhoI site found at 5085 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5392
Illegal XbaI site found at 5884
Illegal SpeI site found at 4329
Illegal PstI site found at 2433
Illegal PstI site found at 2938
Illegal PstI site found at 5386 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5392
Illegal XbaI site found at 5884
Illegal SpeI site found at 4329
Illegal PstI site found at 2433
Illegal PstI site found at 2938
Illegal PstI site found at 5386
Illegal NgoMIV site found at 2821
Illegal NgoMIV site found at 4855
Illegal NgoMIV site found at 6176
Illegal AgeI site found at 2200
Illegal AgeI site found at 3541
Illegal AgeI site found at 9485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 456
Illegal BsaI site found at 5701
Illegal BsaI site found at 7531
Illegal BsaI.rc site found at 5605
Illegal SapI.rc site found at 1108
Illegal SapI.rc site found at 8613
Design Notes
N/A
Source
Please see : http://www.addgene.org/pgvec1?f=c&cmd=findpl&identifier=12247 for the source of this plasmid.